Email updates

Keep up to date with the latest news and content from Journal of Biomedical Science and BioMed Central.

MST logoThe cost of publication in Journal of Biomedical Science is borne by the Ministry of Science and Technology, Taiwan.

Open Access Research

Comparison of microRNA expression in hippocampus and the marginal division (MrD) of the neostriatum in rats

Si Yun Shu1*, Di Qing1, Bin Wang1*, Qi-yi Zeng1, Yan-chen Chen1, Ying Jin2, Chang-chun Zeng2 and Rong Bao3

Author Affiliations

1 Center of Pediatrics, Zhujiang Hospital, Southern Medical University, 510280, Guangzhou, Guangdong, China

2 The College of Biophotonics, South China Normal University, 510631, Guangzhou, Guangdong, China

3 Department of Pediatrics, Sun Yet-San Hospital, Zhong-Shan University, 510120, Guangzhou, China

For all author emails, please log on.

Journal of Biomedical Science 2013, 20:9  doi:10.1186/1423-0127-20-9

Published: 20 February 2013

Abstract

Background

MicroRNAs (miRNAs), a class of highly conserved small non-coding RNA molecules, are known to play essential roles in central nervous system (CNS) by causing post-transcriptional gene silencing. There is much evidence that miRNAs have specific temporal and spatial expression patterns in the mammal brain, but little is known about the role of the region specificity for the gene regulatory networks of the brain. This study represents the first attempt to perform a profiling analysis of the differential expression of miRNAs between hippocampus and the Marginal division (MrD) of the neostriatum in the rat brain.

Results

Microarray was used to detect the expression of 357 miRNAs in hippocampus and the MrD from three rats. A short-list of the most dysregulated 30 miRNAs per rat was generated for data analysis, and the miRNAs that were represented in two or three short-lists were then further analyzed. Quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) was employed to validate the aberrantly expressed miRNAs obtained from the miRNA microarray analysis. A family of 11 miRNAs demonstrated differential expression between the MrD and hippocampus in more than one rat. Amongst these, miR-383 was differentially expressed in all three rats and up-regulated to the largest degree in rat one, and the ten other miRNAs, let-7d*, miR-181b, miR-187, miR-195, miR-214, miR-382, miR-411, miR-466b, miR-592 and miR-1224 were differentially expressed in at least two rats. Of these ten, besides miR-382 and miR-411 which were up-regulated in one rat and down-regulated in another, the other eight miRNAs retained a uniform direction of regulation (up-regulation or down-regulation) between different specimens. When further examined by RT-PCR, the aberrantly expressed miRNAs, except miR-383 and let-7d*, demonstrated differential expression that significantly correlated with the microarray findings.

Conclusion

This study reported that the miRNA expression patterns in MrD was distinct from that of Hip, suggesting the role of miRNAs in the learning and memory function of the MrD probably different from hippocampus.

Keywords:
mRNA transcript; Oligo-microarray; Gene silence; Synapse formation; Learning and memory