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Repairing chronic myocardial infarction with autologous mesenchymal stem cells engineered tissue in rat promotes angiogenesis and limits ventricular remodeling
1 Department of Cardiovascular Surgery, University of Lorraine, Nancy, France
2 School of Surgery, Faculty of Medicine, University of Lorraine, Nancy, France
3 INSERM, U961, University of Lorraine, Nancy, France
4 Department of Nuclear Medicine, Nancyclotep, University of Lorraine, Nancy, France
5 Service de Chirurgie Cardio-vasculaire, CHU-Nancy, Hôpital de Brabois, Allée du Morvan, Vandœuvre Cedex, 54511, France
Journal of Biomedical Science 2012, 19:93 doi:10.1186/1423-0127-19-93Published: 12 November 2012
Tissue engineering scaffold constitutes a new strategy of myocardial repair. Here, we studied the contribution of a patch using autologous mesenchymal stem cells (MSCs) seeded on collagen-1 scaffold on the cardiac reconstruction in rat model of chronic myocardial infarction (MI).
Patches were cultured with controlled MSCs (growth, phenotype and potentiality). Twenty coronary ligated rats with tomoscingraphy (SPECT)-authenticated transmural chronic MI were referred into a control group (n = 10) and a treated group (n = 10) which beneficiated an epicardial MSC-patch engraftment. Contribution of MSC-patch was tested 1-mo after using non-invasive SPECT cardiac imaging, invasive hemodynamic assessment and immunohistochemistry.
3D-collagen environment affected the cell growth but not the cell phenotype and potentiality. MSC-patch integrates well the epicardial side of chronic MI scar. In treated rats, one-month SPECT data have documented an improvement of perfusion in MI segments compared to control (64 ± 4% vs 49 ± 3% p = 0.02) and a reduced infarction. Contractile parameter dp/dtmax and dp/dtmin were improved (p & 0.01). Histology showed an increase of ventricular wall thickness (1.75 ± 0.24 vs 1.35 ± 0.32 mm, p &0.05) and immunochemistry of the repaired tissue displayed enhanced angiogenesis and myofibroblast-like tissue.
3D-MSC-collagen epicardial patch engraftment contributes to reverse remodeling of chronic MI.