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Repairing chronic myocardial infarction with autologous mesenchymal stem cells engineered tissue in rat promotes angiogenesis and limits ventricular remodeling

Pablo Maureira1235*, Pierre-Yves Marie34, Fengxu Yu2, Sylvain Poussier4, Yihua Liu12, Frederique Groubatch2, Aude Falanga2 and Nguyen Tran23

Author Affiliations

1 Department of Cardiovascular Surgery, University of Lorraine, Nancy, France

2 School of Surgery, Faculty of Medicine, University of Lorraine, Nancy, France

3 INSERM, U961, University of Lorraine, Nancy, France

4 Department of Nuclear Medicine, Nancyclotep, University of Lorraine, Nancy, France

5 Service de Chirurgie Cardio-vasculaire, CHU-Nancy, Hôpital de Brabois, Allée du Morvan, Vandœuvre Cedex, 54511, France

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Journal of Biomedical Science 2012, 19:93  doi:10.1186/1423-0127-19-93

Published: 12 November 2012



Tissue engineering scaffold constitutes a new strategy of myocardial repair. Here, we studied the contribution of a patch using autologous mesenchymal stem cells (MSCs) seeded on collagen-1 scaffold on the cardiac reconstruction in rat model of chronic myocardial infarction (MI).


Patches were cultured with controlled MSCs (growth, phenotype and potentiality). Twenty coronary ligated rats with tomoscingraphy (SPECT)-authenticated transmural chronic MI were referred into a control group (n = 10) and a treated group (n = 10) which beneficiated an epicardial MSC-patch engraftment. Contribution of MSC-patch was tested 1-mo after using non-invasive SPECT cardiac imaging, invasive hemodynamic assessment and immunohistochemistry.


3D-collagen environment affected the cell growth but not the cell phenotype and potentiality. MSC-patch integrates well the epicardial side of chronic MI scar. In treated rats, one-month SPECT data have documented an improvement of perfusion in MI segments compared to control (64 ± 4% vs 49 ± 3% p = 0.02) and a reduced infarction. Contractile parameter dp/dtmax and dp/dtmin were improved (p & 0.01). Histology showed an increase of ventricular wall thickness (1.75 ± 0.24 vs 1.35 ± 0.32 mm, p &0.05) and immunochemistry of the repaired tissue displayed enhanced angiogenesis and myofibroblast-like tissue.


3D-MSC-collagen epicardial patch engraftment contributes to reverse remodeling of chronic MI.

Chronic myocardial infarction; Tissue engineering; Mesenchymal stem cell; Ventriculoplasty