There is a major mistake in the order of Figure 5 to Figure 7 in . We replce the Figure 5 and Figure 6 in  with new corrected Figures of Figure 1 and Figure 2. We also replace the correct original order of Figure 6 and Figure 7 in  with Figure 2 and Figure 3 in this correction. Sorry for the inconveniences!
Figure 1. Combination of 0.05% Triton and 0.2 mg/ml heparin give the optimal refolding activities to cleave the synthetic coumarin-labelled peptide substrate, Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2. Panel A: Shows the refolded ZBD activities increased in dose-dependent manner. In the absence of the refolding accessory factors, Triton X-100 and heparin. The significant reduced activities in the high-concentration (> 100 μg/ml) was observed which could be due to autolysis. Panel B: Under 37°C incubation for 18 hours, Triton X-100 and heparin can prevent the activity loss. (All experiments were repeated at two batch of purification and refolding preparation and data collected from a representative experiments)
Figure 2. The polymerization of the 6 kDa ZBD of MMP-7 in pentomer and Octmer demonstrate the significant proteolytic activities towards to the CM-transferrin substrate in CM-transferrin zymographic assay. 300 μg of craboxylmethylated transferrin (CM-transferrin) was co-polymerized with SDS-PAGE as a substrate gel for analyzing the MMP-7 activities in situ
Figure 3. Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 assay for characterization of refolded ZBD. Panel A: Under the optimized conditions, the refolded ZBD shows increasing enzymatic activity in dose-dependent manner. No significant activity loss was found in the high concentration situation. Panel B: approximately 6 ng/ml refolded ZBD shows the increasing activity during the time course study and no significant activity loss during overnight incubation. Panel C: Recombinant ZBD can be inhibited by 10 nM EDTA, 1 mM CoCl2 and synthetic inhibitors, 50 nM BB94 & SC44463 and CoCl2, but not b6 250 nM Phosphoramidon. (All experiments were repeated at two batch of purification and refolding preparation and data collected from a representative experiments)
Yu WH, Huang PT, Lou KL, Yu SS, Lin C: A Smallest 6 kDa Metalloprotease, Mini-matrilysin, in Living World: a Revolutionary Conserved Zinc-Dependent Proteolytic Domain- Helix-Loop-Helix Catalytic Zinc Binding Domain (ZBD).