Table 1

Sequence of primers and conditions used for RT-PCR amplification

Primers

Sequence of primers

PCR amplification conditions

StAR

Sense:5'-CTCAACAACCAAGGAAGGCTGG-3'

94°C, 60 s; 56°C, 40 s;

Atisense:5'-GCAGGTGGGGCCGTGTTCAGC-3'

72°C, 40 s; 30 cycles

3-beta-HSD

Sense:5'-ACTGGCAAATTCTCCATAGCC-3'

94°C, 30 s; 60°C, 45 s;

Atisense:5'-GCTGAACAGTCGACCCTCCTT-3'

72°C, 60 s; 30 cycles

CHOP

Sense:5'-TCTGCCTTTCGCCTTTGAG-3'

94°C, 40 s; 55°C, 60 s;

Atisense:5'-GCTTTGGGAGGTGCTTGTG-3'

72°C, 40 s; 29 cycles

Bip

Sense:5'-CATCAATGAGCCAACAGC-3'

94°C, 45 s; 60°C, 60 s;

Atisense: 5'-AGGTAGAGCGGAACAGG-3'

72°C, 45 s; 26 cycles

PERK

Sense: 5'-GCCGATGGGATAGTGATG-3'

94°C, 45 s; 62°C, 60 s;

Atisense: 5'-GCAGCCTCTACAATGTCTTCT-3'

72°C, 45 s; 28 cycles

GAPDH

Sense:5'-GCTGGGGCTCACCTGAAGG-3'

94°C, 45 s; 56°C, 60 s;

Atisense:5'-GGATGACCTTGCCCACAGCC-3'

72°C, 60 s; 30 cycles

Liu et al. Journal of Biomedical Science 2012 19:4   doi:10.1186/1423-0127-19-4