Figure 5.

Immunofluorescence analysis of PS1 expression in methanol acetone fixed IMR-32 cells after transfection with PS1 siRNA and control siRNA. Plane 1: Non siRNA ransfected IMR-32 Cells; Plane 2: Control siRNA; Plane 3: 50 pmoles PS1siRNA; Plane 4: 75 pmoles PS1 siRNA; Plane 5: 100 pmoles PS1 siRNA. PS1 siRNA and control siRNA transfected IMR-32 cells were probed with PS1 antibody. PS1 siRNA and control siRNA transfected IMR-32 cells were probed with anti PS1 antibody. The signal was revealed using FITC-conjugated secondary antibody and visualized on a fluorescence microscope (o.m. 10X).