The cost of publication in Journal of Biomedical Science is borne by the National Science Council, Taiwan.
Research
Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines
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* Corresponding authors: Lein-Tuan Hou lthou@ha.mc.ntu.edu.tw - Shih-Chieh Hung hungsc@vghtpe.gov.tw
- † Equal contributors
1 Stem Cell Laboratory, Department of Medical Research & Education and Orthopaedics & Traumatology, Veterans General Hospital, Taipei, Taiwan
2 Graduate Institute of Dental Sciences and Department of Periodontology, National Taiwan University, Taipei, Taiwan
3 Graduate Institute of Medical Engineering & Department of Orthopedics, College of Medicine, National Taiwan University, Taipei, Taiwan
4 Institute of Clinical Medicine & Department and Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei, Taiwan
5 Institute of Microbiology and Immunology, National Yang-Ming University, Taipei, Taiwan
Journal of Biomedical Science 2010, 17:64 doi:10.1186/1423-0127-17-64
Published: 29 July 2010Additional files
Additional file 1:
Primer sets and NCBI reference sequence number.
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Additional file 2:
(A) Detection of hTERT mRNA expression in 1C5, 3G11 and 3A6, and (B) Characterization of CD molecules in 3A6. Cytofluorimetric profiles of 3A6 reacted first with (solid line) or without (broken line) mouse MAbs specific for each marker, and second with fluorescein-labeld antimouse Ig antibody.
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Additional file 3:
Box plots show average methylation levels of genes contain CpG islands. P value was calculated using a t-test.
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Additional file 4:
Real-time RT-PCR analysis of expression levels of (A) DNMT1, DNMT3A and DNMT3B, and (B) EZH2 in 3A6 and KP.
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Additional file 5:
(A) RT-PCR analysis of expression levels of hTERT and GAPDH, and (B) Real-time RT-PCR analysis of expression levels of DNMT1, DNMT3A and DNMT3B in primary human mesenchymal stem cells transfected with plasmids carrying control and hTERT vectors. Data are presented as mean ± S.D. *p < 0.01 compared with control as calculated using a t-test.
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