Figure 5.

TNFα regulation by miR-16 and miR-369-3p. Sequence alignment of part of the TNFα 3'UTR and miR-16 or miR-369-3p. The core, non-seed, recognition sequence is shown in bold. (A) TTP is associated with RISC and in a mutually dependent mechanism, TTP and miR-16 promote TNFα mRNA decay through binding to the TNFα ARE. The core non-seed miR-16 recognition motif is shown in bold. (B) miR-369-3p promotes canonical translational suppression or mRNA degradation during normal cellular conditions, but upon cell cycle arrest, FXR1 is associated with RISC to increase translational activity. The core miR-369-3p seed match is shown in bold.