Figure 6.

Knockdown of endogenous Syn-1 by shRNA decreases the numbers of neurites and branches. PC12 (DN-Akt) cells were infected with lentiviral shSyn-1#2 or shControl. (A) Fluorescent microscopic photographs of cells treated with NGF for 5 days. Scale = 50 μm. (B) The number of neurites originating from the neuritogenic cell body. (C) The percentage of branch-bearing neurites. (D) The percentage of neuritogenic cells among GFP-positive cells. Averages and standard deviations are derived from more than ten fields of view. * P < 0.001 and # P < 0.01 (n = 10-15, mean + SD).