Figure 4.

Effect of NaVP on transcriptional regulation of various cardiac development-related genes. Gene expression levels were analyzed using RT-PCR techniques as described in Materials and Methods. (A) A representative RT-PCR analysis of HDAC1, HDAC2, MEF2C, CHF1, GATA4, Nkx2.5 CHF1, Tbx5 and MEF2C mRNA expression in E16 fetal hearts with NaVP treatment on E7. Saline treated mice hearts served as controls. β-actin was used as an internal control. (B) Summary of real-time PCR data from 3 separate experiments. The values from each sample were normalized to that of β-actin mRNA level. Values are presented as means ± SEM of triplicate experiments. * P < 0.05 vs. controls.