Journal of Biomedical Science

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Open Access Research

Cyclic stretch enhances the expression of Toll-like Receptor 4 gene in cultured cardiomyocytes via p38 MAP kinase and NF-κB pathway

Kou-Gi Shyu1,2, Bao-Wei Wang3, Chiu-Mei Lin4 and Hang Chang4*

Author Affiliations

1 Division of Cardiology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan

2 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan

3 School of Medicine, Fu-Jen Catholic University, Taipei, Taiwan

4 Department of Emergency Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan

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Journal of Biomedical Science 2010, 17:15 doi:10.1186/1423-0127-17-15

Published: 5 March 2010

Additional files

Additional file 1:

Supplementary figure. Cyclic stretch increases angiotensin II receptor (Ang II-R) and tumor necrosis factor-α receptor (TNF-α-R) protein expression in cardiomyocytes. (A) Representative Western blots for Ang II-R and TNF-α-R in cardiomyocytes subjected to cyclic stretch by 20% for various periods of time. (B) Quantitative analysis of Ang II-R and TNF-α-R protein levels. The values from stretched cardiomyocytes have been normalized to values in control cells and the data from 4 independent experiments. *P < 0.001 vs. control. **P < 0.05 vs. control. (n = 4 per group).

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