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Research
Involvement of an alternatively spliced mitochondrial oxodicarboxylate carrier in adipogenesis in 3T3-L1 cells
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* Corresponding author: Bing Sun bings@otsuka.com
1 Otsuka Maryland Medicinal Laboratories, Inc, Rockville, Maryland, USA
2 Department of Molecular Pharmacology, Graduate School of Health Biosciences, University of Tokushima, Japan
3 Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, Louisiana, USA
4 Department of Medicine, University of Alabama at Birmingham School of Medicine, Birmingham, Alabama, USA
Journal of Biomedical Science 2009, 16:92 doi:10.1186/1423-0127-16-92
Published: 13 October 2009Additional files
Additional file 1:
Genomic structure and alternative splicing of ODC gene. Schematic diagram of mouse genomic structure and splicing difference between ODC and ODC-AS in the 5' region was shown. The specific forward primers used specifically for ODC and ODC-AS are indicated respectively, as well as the common reverse primer.
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Additional file 2:
Expression of ODC and ODC-AS in murine tissues. PCR amplification of ODC and ODC-AS from cDNAs of murine multiple-tissue panel (Clontech) with primers as shown in Additional file 1, was visualized under UV after being separated on 1% agarose gel containing ethidium bromide. The expected size of the bands was shown on the left and the DNA 1 kb ladder was shown on the right.
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Additional file 3:
Expression of ODC and ODC-AS in the mouse eye. PCR amplification of ODC and ODC-AS from cDNAs of 6 dissected tissues from mouse eye with primers as shown in Additional file 1, was visualized under UV after being separated on 1% agarose gel containing ethidium bromide. The expected size of the bands was shown on the left and the DNA 1 kb ladder was shown on the right.
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