Figure 5.

Mitotic defects in embryos with tlk overexpression are similar to those observed in mars embryos. Embryos with either GFP (GCN4>GFP) or tlk overexpression (GCN4>tlk) at 28.5°C were immunostained with anti-α-Tub (green) and anti-γ-Tub (green) antibodies. The chromosomes were stained by Hoechst 33342 (red). (A) GFP overexpression in metaphase embryo serves as a negative control. (B) Most of the mitotic spindles were normal in metaphase embryos when tlk was overexpressed. A few nuclei showed corrupted mitotic spindles that also appear to have less chromosomal DNA and these are indicated by asterisks. (C) A patch of nuclei with less condensed chromosomes at anaphase is indicated by arrows and the chromosomes seem to remain unseparated. These two phenotypes were also observed in GCN4>tlk embryos at 24°C. The scale bars are 10 μm.