Additional file 1:

Figure S1. Expression of JNK, ERK and p38 MAP kinase in cultured macrophages. (A) Representative Western blot for phosphorylated and total JNK, ERK, and p38 MAP kinase in macrophages after treatment with TNF-α for various periods of time with or without inhibitor. (B) Quantitative analysis of phosphorylated protein levels. The values from treated macrophages have been normalized to matched GAPDH and corresponding total protein measurement and then expressed as a ratio of normalized values to each phosphorylated protein in control cells (n = 3 per group). **P < 0.001 vs. control. *P < 0.01 vs. control. ^‡P < 0.001 vs. 6 hr.

Format: PPT Size: 169KB Download file

This file can be viewed with: Microsoft PowerPoint Viewer

Additional file 2:

Figure S2. Expression of Rac in cultured macrophages. (A) Representative Western blot for phosphorylated and total Rac in macrophages after treatment with mevalonate for various periods of time. (B) Quantitative analysis of phosphorylated protein levels. The values from treated macrophages have been normalized to matched GAPDH and corresponding total protein measurement and then expressed as a ratio of normalized values to each phosphorylated protein in control cells (n = 3 per group).

Format: PPT Size: 185KB Download file

This file can be viewed with: Microsoft PowerPoint Viewer

Additional file 3:

Figure S3. Effect of recombinant resistin and TNF-α on glucose uptake in macrophages. Glucose uptake was measured in macrophages treated for 90 min with 20 μg/mL recombinant mouse resistin or 1 ng/mL TNF-α with or without resistin siRNA or atorvastatin. *P < 0.001. Data are from 3 independent experiments.

Format: PPT Size: 71KB Download file

This file can be viewed with: Microsoft PowerPoint Viewer