Journal of Biomedical Science

official impact factor 1.96

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Open Access Research

Use of serial analysis of gene expression to reveal the specific regulation of gene expression profile in asthmatic rats treated by acupuncture

Lei-Miao Yin1, Gong-Hao Jiang2, Yu Wang1, Yan Wang1, Yan-Yan Liu1, Wei-Rong Jin3, Yu-Dong Xu1, Qing-Hua Zhang3 and Yong-Qing Yang1*

Author Affiliations

1 Shanghai Research Institute of Acupuncture and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai, PR China

2 National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology & Ecology, Chinese Academy of Sciences, Shanghai, PR China

3 National Engineering Center for Biochips at Shanghai, Shanghai, PR China

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Journal of Biomedical Science 2009, 16:46 doi:10.1186/1423-0127-16-46

Published: 6 May 2009

Additional files

Additional file 1:

Rat on the suspended shelf. For the convenient manipulation of the acupuncture points on the back, rat was placed on the suspended shelf, which could easily make it calm and stand still without anesthesia.

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Additional file 2:

The intron-spanning primer pairs of genes of quantitative real-time PCR confirmation. The primer pairs of 3 differentially expressed genes of interest (Dusp1, S100A9, and MT-2) and the reference gene (GAPDH).

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Additional file 3:

The comparisons of Cdyn and RR of the four groups. The table included the 10 min measurement of Cdyn and RR of the four groups.

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Additional file 4:

The lists of differentially expressed tags of PCK/AS, PAS/ASAC, PCK/CKAC. The details of the 186, 130 and 144 differentially expressed tags (P < 0.05) of the PCK/AS, PAS/ASAC, and PCK/CKAC. This table included the tag sequence, tag copies of different libraries, UniGene number and the gene description.

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Additional file 5:

The gene lists of DAVID gene functional classification of PCK/AS, PAS/ASAC, PCK/CKAC. The complete gene lists of 6 functional groups with enrichment scores equal to or higher than 1 were shown for the Figure 4.

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Additional file 6:

The gene lists of the KEGG pathways. The complete gene lists of the matched KEGG pathways were demonstrated for the Table 3.

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Additional file 7:

The gene lists of Venn diagram. The details of the different gene groups of Venn diagram, which included the tag sequence, tag copies of different libraries, UniGene number and the gene description.

Format: XLS Size: 28KB Download file

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Open Data