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Research

Use of serial analysis of gene expression to reveal the specific regulation of gene expression profile in asthmatic rats treated by acupuncture

Lei-Miao Yin¹ , Gong-Hao Jiang² , Yu Wang¹ , Yan Wang¹ , Yan-Yan Liu¹ , Wei-Rong Jin³ , Yu-Dong Xu¹ , Qing-Hua Zhang³ and Yong-Qing Yang¹

¹  Shanghai Research Institute of Acupuncture and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai, PR China

²  National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology & Ecology, Chinese Academy of Sciences, Shanghai, PR China

³  National Engineering Center for Biochips at Shanghai, Shanghai, PR China

author email corresponding author email

Journal of Biomedical Science 2009, 16:46doi:10.1186/1423-0127-16-46

Published:	6 May 2009

Abstract

Background

Asthma has become an important public health issue and approximately 300 million people have suffered from the disease worldwide. Nowadays, the use of acupuncture in asthma is increasing. This study intended to systematically analyze and compare the gene expression profiles between the asthmatic and acupuncture-treated asthmatic rat lung, and tried to gain insight into the molecular mechanism underlying the early airway response (EAR) phase of asthma treated by acupuncture.

Methods

Four tag libraries of serial analysis of gene expression (SAGE) were established from lung tissues of control rats (CK), asthmatic rats (AS), asthmatic rats treated by acupuncture (ASAC), and control rats treated by acupuncture (CKAC). Bioinformatic analyses were carried out by using the methods including unsupervised hierarchical clustering, functional annotation tool of the database for annotation, visualization, and integrated discovery (DAVID), gene ontology (GO) tree machine, and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis.

Results

There were totally 186 differentially expressed tags (P < 0.05, P_CK/AS) between the libraries of CK and AS, 130 differentially expressed tags between libraries of AS/ASAC (P < 0.05, P_AS/ASAC), and 144 differentially expressed tags between libraries of CK/CKAC (P < 0.05, P_CK/CKAC). The gene expression profiles of AS and ASAC were more similar than other libraries via unsupervised SAGE clustering. By comparison of P_CK/AS and P_AS/ASAC, the DAVID genes functional classification was found to be changed from "immune response" to "response to steroid hormone stimulus", and the GO term "antigen processing and presentation of peptide antigen" disappeared in P_AS/ASAC. Totally 3 same KEGG pathways were found among the three groups. Moreover, 21 specific tags of the acupuncture in treating asthma were detected using Venn diagrams.

Conclusion

Our SAGE research indicates that the gene expression profile of the EAR phase of asthma could be effectively and specifically regulated by acupuncture, which suggests that the gene expression of immune response and steroid hormone may play an important role in the treatment.