Figure 4.

In vitro interaction of GAPDH with plus-strand (A) and minus-strand (B) of JEV riboprobes. Lane 1, free probe (F). A. GAPDH (50 ng, 200 ng, 1 μg; lanes 2–4), or BSA (1 μg; lane 5) were added to the binding reaction as indicated on the top. B. GAPDH proteins (55 ng, 165 ng, 550 ng) without (lanes 2–4) or with 70 μM of H₂O₂ treated (lanes 5–7) were added to the binding reaction. RNA-protein complexes (RPC) are indicated by arrows.