Table 7

Real Time amplification of Muscarinic M1 receptor mRNA from the Corpus striatum of Control, Diabetic, and Diabetic+Insulin treated group rats

Experimental group

RQ Value


Control

0


Diabetic

3.14 ± 0.25**


Diabetic + Insulin treated

0.86 ± -0.66ψψ


Values are mean ± S.D of 4–6 separate experiments. Each group consist of 6–8 rats Relative Quantification values and standard deviations are shown in the table. The relative ratios of mRNA levels were calculated using the ΔΔCT method normalized with β-actin CT value as the internal control and Control CT value as the calibrator.

** p < 0.01 when compared with control

ψψp < 0.01 when compared with diabetic group

Gireesh et al. Journal of Biomedical Science 2009 16:38   doi:10.1186/1423-0127-16-38

Open Data